Composite

Part:BBa_I4285

Designed by: Austin Che   Group: Antiquity   (2004-10-02)


Trans-splicing Tetrahymena ribozyme to remove LVA tag

Designed ribozyme to remove LVA tag from constructs built by Reshma containing a LVA tag on the YFP gene. Replace the LVA tag with a stop codon to allow for higher expression.


Usage and Biology

The ribozyme consists of the native sequence from the intron located in the Tetrahymena 26S rRNA from 28-414 (standard numbering).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 430
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 332
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/composite/uncategorized
Parameters
None